Species: Nile tilapia (Oreochromis niloticus)

Country of 1st Author: China
Year: 2014

Institute	Southwest University 400715 Chongqing CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	The guide RNA (gRNA) and Cas9 mRNA were coinjected into one-cell-stage embryos of the XY tilapia with the optimal concentrations of 50 ng/µl and 500 ng/µl, respectively.
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
dmrt6 doublesex and mab-3-related transcription factor 6	helps coordinate the transition of male germ cells from mitosis to meiosis	Reproduction	CRISPR Cas9 (spCas9)	Dmrt6 knockout by CRISPR/Cas9

References, Documents, and Links

• Isolation of Doublesex- and Mab-3-Related Transcription Factor 6 and Its Involvement in Spermatogenesis in Tilapia