Species: Nile tilapia (Oreochromis niloticus)

Country of 1st Author: China
Year: 2016

Institute	Southwest University 400715 Chongqing CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Fertilized eggs (XX and XY) were divided into a control and a microinjected group. The microinjected groups were coinjected into the one-cell stage embryos with gRNA and Cas9 mRNA
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
Sf-1 Steroidogenic factor-1	Reproduction and Development. Gonadal dysgenesis and reduced steroidogenic cells in XX and XY fish.	Reproduction	CRISPR Cas9 (spCas9)	To minimize the risk that phenotypes described result from off-target sites, 2 target sites were selected in different region of the fourth exon of sf-1. The 2 cutting sites were targeted separately rather than simultaneously to result in a specific excision

References, Documents, and Links

• Haploinsufficiency of SF-1 Causes Female to Male Sex Reversal in Nile Tilapia, Oreochromis niloticus