Species: Nile tilapia (Oreochromis niloticus)

Country of 1st Author: Israel
Year: 2021

Institute	Volcani Institute IL
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Microinjection of RNPs
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
slc45a2 solute carrier family 45 member 2		Colour	CRISPR Cas9 (spCas9)	Ribonucleoprotein heterocomplexes of CRISPR/Cas9. Mostely in multiplex for targeting of multiple sites. Induction by microinjection into zygotes at single-cell stage.

References, Documents, and Links

• Genome Editing Using the CRISPR-Cas9 System to Generate a Solid-Red Germline of Nile Tilapia (Oreochromis niloticus)