Species: Nile tilapia (Oreochromis niloticus)

Country of 1st Author: China
Year: 2020

Institute	Southwest University 400715 Chongqing CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Artificially synthesized gRNA and Cas9 mRNA were co-injected into one-cell stage embryos
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
IGF3 insulin-like growth factor 3	Insulin-like growth factor	Reproduction	CRISPR Cas9 (spCas9)	a gRNA target site containing a restriction enzyme site was selected by identifying sequences corresponding to GGN18NGG on the sense or antisense strand of igf3 using the online tool, ZIFIT Targeter

References, Documents, and Links

• Regulation of spermatogenesis and reproductive capacity by Igf3 in tilapia