Species: Nile tilapia (Oreochromis niloticus)

Country of 1st Author: China
Year: 2016

Institute	Southwest University 400715 Chongqing CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	The guide RNA and Cas9 mRNA were co-injected into one-cell-stage embryos
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
gsdf Gonadal soma-derived factor		Reproduction	CRISPR Cas9 (spCas9)	A guide RNA site containing BstNI adjacent to proto-spacer adjacent motif in the first exon of gsdf was selected for mutation analysis

References, Documents, and Links

• gsdf is a downstream gene of dmrt1 that functions in the male sex determination pathway of the Nile tilapia