Species: Nile tilapia (Oreochromis niloticus)

Country of 1st Author: China
Year: 2020

Institute	Southwest University 400715 Chongqing CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	gRNA and Cas9 mRNA were co-injected into one-cell stage embryos
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
foxh1 forkhead box H1	Reproduction and Development. The foxh1-/- XX fish showed oogenesis arrest and a significantly lower gonadal:somatic index.	Reproduction	CRISPR Cas9 (spCas9)	The gRNA target sequence containing a HaeIII restriction site adjacent to the protospacer adjacent motif (PAM) sequence was located in the second exon of foxh1

References, Documents, and Links

• Homozygous mutation of foxh1 arrests oogenesis causing infertility in female Nile tilapia