Species: Nile tilapia (Oreochromis niloticus)

Country of 1st Author: China
Year: 2020

Institute	Southwest University 400715 Chongqing CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Embryos at the one-cell stage were microinjected with gRNA and Cas9 mRNA
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
Cyp11c1 cytochrome P450, family 11, subfamily c, polypeptide 1	The Cyp11c1 is a fish gene encoding a CYP450 enzyme, which was originally found in Zebrafish (Danio rerio), this enzyme mainly catalyze the formation of cortisol and 11-Ketotestosterone (11-KT)	Reproduction	CRISPR Cas9 (spCas9)	The target site in the coding region of the first exon of cyp11c1 was selected with an online software package

References, Documents, and Links

• Loss of Cyp11c1 causes delayed spermatogenesis due to the absence of 11-ketotestosterone