Species: Nile tilapia (Oreochromis niloticus)

Country of 1st Author: United States
Year: 2015

Institute	Southwest University 400715 Chongqing CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	About 500 pg of the gRNA (150 ng/µl) and Cas9 mRNA (500 ng/µl), mixed at a molar ratio of 1:1, was microinjected directly into XX or XY fertilized eggs.
Remarks	Two gRNA target sites were selected for each gene on the sense or antisense strand of amh/amhy, amhrII and gsdf with ZIFIT Targeter.
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
AMH anti-Mullerian hormone	The AMH gene provides instructions for making a protein that is involved in male sex differentiation.	Reproduction	CRISPR Cas9 (spCas9)
amhΔ-y		Reproduction	CRISPR Cas9 (spCas9)
amhy Y-localised anti-Müllerian hormone	Master sex-determining gene	Reproduction	CRISPR Cas9 (spCas9)
AMHR2 anti-Müllerian hormone receptor type 2	The AMHR2 gene provides instructions for making the anti-Müllerian hormone (AMH) receptor type 2, which is involved in male sex differentiation.	Reproduction	CRISPR Cas9 (spCas9)

References, Documents, and Links

• A Tandem Duplicate of Anti-Müllerian Hormone with a Missense SNP on the Y Chromosome Is Essential for Male Sex Determination in Nile Tilapia, Oreochromis niloticus