Species: Nile tilapia (Oreochromis niloticus)

Country of 1st Author: United States
Year: 2022

Institute	Shanghai Fisheries Institute 265 Jiamusi Road, Shanghai, 200433 CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Five hundred one-cell fertilized eggs from two golden tilapia crosses were injected with pMDS-PMEL plasmid and Ac transposase mRNA.
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
Pmel17 pre-melanosomal protein	Color change	Colour	CRISPR Cas9 (spCas9)	nockout of Pmel17 with CRISPR/Cas9 in blackish tilapias resulted in golden coloration, and rescue of Pmel17 in golden tilapias recovered the wild-type blackish color, indicating that Pmel17 is the gene determining the golden and blackish color

References, Documents, and Links

• The Insertion in the 3' UTR of Pmel17 Is the Causal Variant for Golden Skin Color in Tilapia