Performance and Stability of Scab Resistance Gene from Barley on Transgenic Apple Lines
January 28, 2011| |
Scab, caused by Venturia inequalis, is the most prominent fungal disease of apple in Europe. To prevent infestation, growers use fungicides, with 15-20 sprayings per season. To reduce the use of fungicides, Frans Krens of the Wageningen University and Research Center together with other scientists, introduced hordothionin gene from barley to elite apple cultivars Elstar and Gala. To test the performance and stability of the gene, they planted genetically modified (GM) hth lines together with GM apple without hth, non-GM susceptible, and non-GM resistant controls arranged randomly in a field trial for four years. They recorded scab resistance after artificial inoculation of V. inequalis in the first year and natural infestation in the following years. RT-PCR was used to check level of scab resistance and results showed that four out of the six GM hth lines are significantly less susceptible to scab during the entire four years of trial.
Read the open-access research article published by Transgenic Research journal at http://www.springerlink.com/content/jxm15574n14t0209/.
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