CRISPR-Cas9 Used to Study Genes Involved in Grapevine Disease SymptomsMay 13, 2020
Texas Tech University researchers used CRISPR-Cas9 technology in grapevine (Vitis vinifera) to elucidate the functions of specific genes in Pierce's disease and Grapevine Red Blotch Virus disease symptoms. The findings are released in Transgenic Research.
Pierce's disease is caused by the Xylella fastidiosa bacterium through xylem sap-sucking insects as vectors, while Grapevine Red Blotch Virus causes Red Blotch Disease and is transmitted by alfalfa leafhopper Spissistilus festinus. The effect of anthocyanin levels in specific tissues of grapevine on PD and GRBV disease symptoms remains unclear, and thus an investigation was conducted focusing on Trans-Acting Small-interfering locus4 (TAS4) and MYBA5/6/7 transcription factor.
Transgenic grape plants targeting TAS4b and MYBA7 were generated using CRISPR-Cas9 technology. There were no visible anthocyanin accumulation phenotypes observed in regenerated plants, possibly due to the presence of genetically redundant TAS4c and MYBA5/6 loci or absence of inductive environmental stress conditions.
Read more results in Transgenic Research.
You might also like:
- Pocket K No. 54: Plant Breeding Innovation: CRISPR-Cas9
- Researchers Use CRISPR for Apple and Grapevine Improvement
- Scientists Investigate Grapevine's Population Genetics Using Structural Variants
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
- Scientists Clone SARS-CoV-2 Genome Using Quick Yeast-Based Method
- COVID-19 Treatment Efforts Using Plant Technologies
News from Around the World
- Global Demand for Cotton Declines Due to COVID-19 Crisis
- Bolivia to Speed Up Evaluation of GM Crops
- Review Shows Biotechnological Strategies to Develop Drought Resistant Crops
- Plants Have Memories, But How Do Plants Forget?
- Scientists Discover Plant Molecular "Alarm" System Protects them from Predators
- Comparative Genomics Helps Reduce Spread of Bacterial Canker in Tomatoes
Plant Breeding Innovations
- CRISPR-Cas9 Used to Study Genes Involved in Grapevine Disease Symptoms
- International Plant Biotech Experts Weigh in on New Breeding Technology Regulations
Subscribe to CBU: