Researchers Use CRISPR for Apple and Grapevine ImprovementNovember 7, 2018
Important fruit crops apple and grapevine are subjects for crop improvement by conferring biotic and abiotic stress tolerance through traditional breeding and modern biotechnology. As approvals for field testing and commercialization have been limited in most crops, researcher Yuriko Osakabe from Tokushima University in Japan and colleagues explore genome editing to genetically improve apple and grapevine.
In their article in Nature, the group of researchers described two protocols, namely, plasmid-mediated genome editing and direct delivery of CRISPR-Cas9 ribonucleoproteins for genome editing of apple and grapevine. They transformed CRISPR-Cas9 components into protoplasts and verified mutations produced by the system. Results showed high efficiency and accuracy of the system in the two fruit crops, taking 2-3 weeks for genetic transformation of the CRISPR-Cas9 system and more than three months for plant regeneration and verification of the genome edits.
For more information, read the article in Nature Protocols.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- 13 WTO Members Support Policy Approaches to Enable Innovation in Agriculture
- Farmers and University Students Call for Urgent Action on Biotech Legislation in Uganda
- US Patent Awarded to DNA-Targeting Complex
- High Temperatures Can Trigger a Reaction in a Plant's RNA
- ASTA Lauds International Statement Supporting of Plant Breeding Innovation
- Scientists Discover Gene Regulator that Allows Plant to Rehydrate After Drought
- FAS Jakarta Launches "Biotech Ambassadors" Outreach
- USDA: The Philippines Remains as Asia's Leader in Biotech
- Gene Discovery Could Pave Way for Disease Resistant Crops
- Small Genetic Differences Make Plants into Better Teams
- Agriculture Minister of The Netherlands Opens Door to Genetic Modification
- Protoplast Isolation Method for Genetic Improvement of Pineapple
- Gene from Castor Bean Increases Unusual Oil in Camelina
Plant Breeding Innovations
- Researchers Use CRISPR for Apple and Grapevine Improvement
- Plasmid-Free Genome Editing of Cabbage and Chinese Cabbage
- Plant Genome Editing Database (PGED) Goes Live
Beyond Crop Biotech
- Scientists Reveal a Gene Related to Alzheimer's Disease Using CRISPR
- Pocket K: Contributions of Agri-biotech in Alleviation of Poverty and Hunger (Updated)
Subscribe to CBU: