CRISPR-Cas9 Used in Targeted Nucleotide Substitution in Rice Blast Fungus
May 29, 2019| |
Genome editing using CRISPR-Cas9 has been one of the promising techniques used in genetic engineering. Scientists from Meiji University developed a new genome editing strategy via single crossover-mediated homologous recombination in rice blast fungus, which involves the CRISPR-Cas9 system.
With the use of the new technique, highly efficient and freely programmable base substitutions within the desired genomic locus, and target gene disrupted mutants were successfully obtained. Furthermore, the new method allowed one-step GFP gene knock-in at the C-terminus of the targeted gene.
The researchers concluded that the single cross-over mediated genome editing strategy can increase the scope of the genome editing technology.
Read the research article in Scientific Reports.
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