Crop Biotech Update

Scientists Develop Transgenic Tomatoes with Resistance to Root Knot Nematode

March 27, 2019
Root knot nematode is one of the serious problems in growing solanceous crops including tomato. Thus, scientists from the University of Agricultural Sciences in India explored to develope tomato's resistance against root knot nematode through genetic engineering

Lectin genes from a perennial herb (Remusatia vivipara) and soil-borne fungus (Sclerotium rolfsii) were introduced into tomato using Agrobacterium tumefaciens transformation. Polymerase chain reaction showed that 101 rvl1 and srl1-transformed plants exhibiting kanamycin resistance carry transgenes with 4.59% transformation efficiency. Mendelian segregation of the introduced genes was observed in the first generation of progenies. Three promising events of rvl1 and srl1 were selected and grown then exposed to root knot nematode. High levels of resistance were observed in the transgenic plants in comparison with the non-transformed control. 

These results indicate that the technology can be used for variety development of root knot nematode resistant tomatoes.

Read the research article in Transgenic Research.