News and Trends

http://www.biotechnologyforbiofuels.com/content/pdf/1754-6834-3-2.pdf

Acid-pretreatment of lignocellulosic biomass for ethanol production usually destroys the tight lignin-cellulose matrix of the biomass, and results in the liberation of simple sugars which can then be yeast-fermented to ethanol. There are undesirable by-products, though, that can be produced by acid pretreatment, (depending on the reaction conditions used). These by-products can inhibit ethanol fermentation in yeasts. "Furan aldehydes" ("furfural" and "hydroxymethyl furfural (HMF)", from the degradation of xylose and glucose sugars, respectively) are major fermentation inhibition by-products from the acid pretreatment process. Scientists from Central Michigan University, John Hopkins University, and the Agricultural Research Service, Department of Agriculture (all in the United States), investigated the physiological/biochemical ethanol-inhibition effects of furfural (in the yeast, Saccharomyces cerevisiae). They found that "furfural was shown to cause cellular damage that is consistent with ROS (reactive oxygen species) accumulation in cells, which include damage to mitochondria and vacuole membranes, the actin cytoskeleton, and nuclear chromatin". Furfural concentrations of about 25 mM could allow repair response mechanisms for the (growth and fermentation) recovery of yeasts (after a long lag phase). Details of their study are published in the open access journal, Biotechnology for Biofuels (URL above)..


http://www.biofuelsdigest.com/blog2/2010/01/18/masdar-institute-boeing-etihad-airways-and-uop-honeywell-announce-sustainable-bioenergy-research-project-in-uae-focused-on-salicornia-as-aviation-biofuel/
http://www.biofuels-news.com/industry_news.php?item_id=1639
http://www.uop.com/pr/releases/SBRP%20Masdar%20Agreement%20PR%20-%20FINAL.pdf

The Masdar Institute of Science and Technology (Abu Dhabi), together with UOP LLC (a Honeywell Company), Boeing and Etihad Airways, recently announced the establishment of the Sustainable Bioenergy Research Project (SBRP) aimed at "developing sustainable bioenergy solutions". The SBRP program will involve "research projects in the arid salt-rich environment in Abbu Dhabi that will feature innovative and promising saltwater farming practices. Integrated saltwater agricultural systems will be used to support the development and commercialization of aviation biofuels. The UOP website describes the integrated approach as one which "uses saltwater to create an aquaculture-based farming system in parallel with the growth of mangrove forests and Salicornia, a plant that thrives in salty water. These biomass sources can be sustainably harvested and used to generate clean energy, aviation biofuels and other products". The Masdar Institute will host the SBRP and provide laboratory and demonstration facilities..

Energy Crops and Feedstocks for Biofuels Production

http://www.biofuelsdigest.com/blog2/2010/01/25/researchers-aim-for-high-sugar-biofuel-feedstock-from-mapping-sunflower-genome-crossing-silverleaf-with-common-sunflower/
http://www.cleanskies.com/articles/doe-funds-sunflower-power-research

The United States Department of Energy (US-DOE) is joining with the US Department of Agriculture, Genome Canada and France's National Institute for Agricultural Research to fund a project worth $10.5 million, aimed at mapping the DNA sequence of the sunflower plant. Using the latest genotyping and sequencing technologies, the sunflower genome will be sequenced, and genes for agriculturally important traits (such as oil content in seeds) will be located. A high oil-yielding sunflower plant is a potential feedstock for biodiesel production. The development of a hybrid variety of sunflower, grown as a dual-use crop, is one of the potential applications of the research. According to (University of British Columbia-based) project leader, Dr. Loren Rieseberg, "The seeds would be harvested for food and oil, while the stalks would be utilized for wood or converted to ethanol. As a dual-use crop it wouldn't be in competition with food crops for land"..

Biofuels Processing

http://www.nature.com/nature/journal/v463/n7280/pdf/nature08721.pdf
(may require paid subscription for full access to paper) https://www.orau.gov/gtl2010/abstracts/Keasling_Jay_06.pdf

Biodiesel is commonly produced through a series of steps involving the cultivation of a high-oil producing bioenergy crop, extraction of the crop's oil, and application of chemical processing steps. The chemical steps usually entail high energy and processing costs. Recent research could make the biomass to biodiesel conversion route less costly. A collaborative research group of the United States Department of Energy (US-DOE)-Joint BioEnergy Institute (JBEI), recently reported the development of an E. coli bacterium which can convert biomass directly to biodiesel, and other fatty-acid-derived chemicals. Using the tools of synthetic biology, they first diverted fatty acid metabolism toward the production of fuels and other chemicals from glucose. Then they engineered the new E.coli strain to produce hemicellulases (enzymes for the conversion of hemicellulose from plant biomass into simple sugars). A complete production scheme has been reportedly demonstrated, but strategies to achieve "increases in titer, productivity and yield" are needed for industrial transition. Details of the study appears in the journal, Nature (URL above)..


http://www.pnas.org/content/106/51/21550.abstract?sid=870ef4d7-2491-4c4a-bd3c-b9a9725e0666
(may require paid subscription for complete access to paper)
http://researchstories.asu.edu/stories/self-destructing-bacteria-improve-biofuel-production-1350

Seed oils from cultivated plants have long been used as raw material sources for biodiesel production. Recently, however, attention has shifted to the use of microorganisms (particularly photosynthetic microorganisms) as potential oil sources for biodiesel production. One such type of microorganism under study is a class of photosynthetic bacteria, called "cyanobacteria". Since the oil produced by these microorganisms is generally an intracellular product, the cell walls have to be destroyed by application  of physical or chemical means (such as ultrasound, high pressure, or use of chemicals) Cell wall disruption often entails a high oil recovery cost. Researchers from the Arizona State University (ASU, United States) have developed a way to reduce the cost of oil recovery from cyanobacteria, by programming it to "self-destruct". The programmed autolysis is induced by the addition of nickel; autolysis eventually liberates the intracellularly-trapped oil without further addition of physical or chemical recovery processes. The ASU press release gives an overview of the method: "The genes were taken from a mortal bacterial enemy, called a bacteriophage, which infect the bacteria, eventually killing the microbes by causing them to burst like a balloon. The scientists swapped parts from bacteriophages that infect E. coli and salmonella, simply added nickel to the growth media, where the inserted genes produced enzymes that slowly dissolved the cyanobacteria membranes from within". The full paper is recently published in the Proceedings of the National Academy of Sciences (PNAS) (URL above)..

Biofuels Policy and Economics

http://www.biofuels-news.com/content_item_details.php?item_id=260

The Biofuels International website reports that biofuel ethanol production in the European Union (EU) in 2008 increased by 58% compared to the previous year. The major feedstock used is reported to be from cereals. About 63% of the 2.8 billion liters of ethanol produced in 2008 used wheat as the cereal feedstock. Ethanol from maize accounted for 27% , while rye and barley accounted for 4.3% and 3.5% of production, respectively. Triticale (a hybrid of wheat and rye) is also reported as a marginal ethanol feedstock used in Germany.