Virus-Resistant GM Common Bean from RNAi
June 15, 2007| |
RNA interference (RNAi) was used to obtain a common bean line resistant to bean golden mosaic virus (BGMV), the virus responsible for golden mosaic disease in the crop. The research, conducted in Brazil, reports that 93% of the plants from the transgenic resistant line were free of symptoms upon high pressure inoculation.
BGMV is a major constraint in bean production that causes yield losses between 40 to 100%. The virus is transmitted by the whitefly Bemisia tabaci. The RNAi approach uses an RNA interference construct to silence the sequence region of the AC1 viral gene, producing resistant common bean. Compared to the non-transgenic control with 100% golden mosaic incidence after 38 days of inoculation, the transgenic line has only 7.8% disease incidence.
For details the complete paper published in Molecular Plant-Microbe Interactions can be accessed by subscribers at http://dx.doi.org/10.1094/MPMI -20-6-0717.
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