New Gene Editing Tool to Enable Insertion of Large Sections of DNA

Researchers from Cornell University and McGill University have developed a new gene editing tool that could improve the accuracy and efficiency of genetic modifications in plants, animals, and humans. The technique, called an RNA-guided transposition system, uses engineered bacterial components to insert large sections of DNA into specific locations in a genome. Published in Molecular Cell, the study introduces an alternative to existing CRISPR-Cas systems that could support research, crop improvement, and development of treatments for genetic diseases that require changes to larger DNA sequences.

The system combines two bacterial proteins, TldR and TniQ, to identify the target location and guide the precise insertion of genetic material. Unlike base and prime editing, which modify short sequences, the new technique can potentially insert entire DNA segments to address more complex genetic conditions. The technology's size may also make it easier to deliver into cells using viruses. The research team has filed a patent and will explore related proteins to establish further applications.

For more information, read the news article from Cornell University.