Enhanced Lipid Production via Expression of AtWRI1 Transcription Factor in Microalgae
October 25, 2017https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-017-0919-5
| |
Genetic engineering of microalgae is necessary to produce economically important strains for biofuel production. Transcription factors (TFs) are emerging as good alternatives for engineering of microalgae, to increase production of biomaterials as well as to enhance stress tolerance.
The team of Nam Kyu Kang from Korea Advanced Institute of Science and Technology investigated the Wrinkled1 TF in Arabidopsis (AtWRI1), by expressing it in the industrial microalgae, Nannochloropsis salina designated as NsAtWRI1. The Wrinkled1 TF gene is a known regulator of lipid biosynthesis in plants.
Results revealed that the total lipid contents and the fatty acid methyl ester yields (FAME) were greater in NsAtWRI1 transformants than in wild types under both normal and stress conditions. Further analysis indicated that the AtWRI1 transcription factor upregulated genes involved in lipid synthesis in N. salina, resulting in enhanced lipid production in the NsAtWRI1 transformants from early growth phase.
These suggest that expression of AtWRI1 TF can be used for efficient biofuel production in industrial microalgae.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Nigeria Prepares for Commercial Release of Bt Cowpea and Bt Cotton in 2018
- Disease Resistant Potato Field Tests Show Positive Results in Uganda
- U.S. Lawmakers Call for Consistency in Regulating Biotech
- International Research Team Releases New Version of Sunflower Genome
- Scientists Discover Path to Improved Barley Grain Quality
- EFSA Releases Risk Assessment of GM Maize GA21 for Renewal of Authorization
- Rice 'Supercharged' with Maize Gene May Help Boost Yields
-
Research Highlights
- Confined Expression of Bt Protein in Insect Wounding Parts of Potato Plants
- Host-Induced Gene Silencing of PsCPK1 in Pathogen Enhances Stripe Rust Resistance in Wheat
-
Beyond Crop Biotech
- Stevia Plant Genome Sequence Revealed for the First Time
- CAS Scientists Develop Low-Fat Pigs
-
Announcements
- BIO-Europe 2017
-
Resources
- Biotech Country Facts and Trends
-
Plant
- Application of CRISPR-CAs9 in Chrysanthemum Using Transgenes as Targets
- CRISPR-Cas9-Mediated Gene Replacement Generates Long-shelf Life Tomato Lines
-
Read the latest: - Biotech Updates (May 8, 2024)
- Gene Editing Supplement (April 24, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet