Developer:
DuPont (Pioneer Hi-Bred International Inc.)

Method of Trait Introduction:
Conventional breeding - cross hybridization and selection involving transgenic donor(s)

GM Trait s :
Glufosinate herbicide tolerance , Glyphosate herbicide tolerance , Coleopteran insect resistance , Lepidopteran insect resistance , Mannose metabolism

Commercial Trait:
(Stacked)  Herbicide Tolerance + Insect Resistance

Summary of Basic Genetic Modification

Gene Introduced	Gene Source	Product	Function
cry1Fa2	synthetic form of cry1F gene derived from Bacillus thuringiensis var. aizawai	modified Cry1F protein	confers resistance to lepidopteran insects by selectively damaging their midgut lining
cp4 epsps (aroA:CP4)	Agrobacterium tumefaciens strain CP4	herbicide tolerant form of 5-enolpyruvulshikimate-3-phosphate synthase (EPSPS) enzyme	decreases binding affinity for glyphosate, thereby conferring increased tolerance to glyphosate herbicide
pat	Streptomyces viridochromogenes	phosphinothricin N-acetyltransferase (PAT) enzyme	eliminates herbicidal activity of glufosinate (phosphinothricin) herbicides by acetylation
cry34Ab1	Bacillus thuringiensis strain PS149B1	Cry34Ab1 delta-endotoxin	confers resistance to coleopteran insects particularly corn rootworm by selectively damaging their midgut lining
cry35Ab1	Bacillus thuringiensis strain PS149B1	Cry35Ab1 delta-endotoxin	confers resistance to coleopteran insects particularly corn rootworm by selectively damaging their midgut lining
cry1Ab	Bacillus thuringiensis subsp. kurstaki	Cry1Ab delta-endotoxin	confers resistance to lepidopteran insects by selectively damaging their midgut lining
mcry3A	synthetic form of cry3A gene from Bacillus thuringiensis subsp. tenebrionis	modified Cry3A delta-endotoxin	confers resistance to coleopteran insects particularly corn rootworm pests by selectively damaging their midgut lining
pmi  *	Escherichia coli	Phosphomannose Isomerase (PMI) enzyme	metabolizes mannose and allows positive selection for recovery of transformed plants

 * : Selection Marker/Reporter  

Summary of Regulatory Approvals: Country, Year and Type of Approval

Country	Food direct use or processing	Feed direct use or processing	Cultivation domestic or non-domestic use
Canada
European Union
Japan
Mexico
Philippines
South Korea
Taiwan

 

Regulatory and Biosafety Information

• Japan Biosafety Clearing House
• Mexico CIBIOGEM
• Philippines DA - BPI Biosafety
• Taiwan Food and Drug Administration

Detection Methods

• EU Reference Laboratory for GM Food and Feed
• EU Reference Laboratory for GM Food and Feed
• EU Reference Laboratory for GM Food and Feed
• EU Reference Laboratory for GM Food and Feed
• EU Reference Laboratory for GM Food and Feed