Species: Goat (Capra hircus)

Country of 1st Author: China
Year: 2017

Institute	Nanjing Agricultural University Nanjing, Jiangsu CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	one-cell embryos were then co-injected with sgRNAs and different concentrations ofCas9 mRNA by cytoplasmic injection.
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
BLG β-Lactoglobulin	Knockout of beta-lactoglobulin for hypoallergenic milk	Hypoallergenic	CRISPR Cas9 (spCas9)	co-injection of Cas9 mRNA and small guide RNAs (sgRNAs) into goat embryos at the one-cell stage

References, Documents, and Links

• Generation of beta-lactoglobulin knock-out goats using CRISPR/Cas9