Species: Silkworm (Bombyx mori)

Country of 1st Author: China
Year: 2023

Institute	Southwest University 400715 Chongqing CN
Editing Method	Embryo edit
SDN Type	SDN-3
Delivery	Let-7-gRNA transgenic vector was microinjected into G0 embryos, and the positive G1 broods were screened in day 6 embryos by examining the expression of green fluorescence protein (GFP) in the eyes
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
let-7 lethal-7	Improved larval and silk gland growth	Yield	CRISPR Cas9 (spCas9)	Fresh eggs were microinjected with pBac[3 × P3-EGFP, U6-let-7-gRNA] and the pHA3pig helper vector, with the final concentration of each plasmid being 300 ng/µL.

References, Documents, and Links

• CRISPR/Cas9-mediated gene editing of the let-7 seed sequence improves silk yield in the silkworm, Bombyx mori