Species: Sheep (Ovis aries)

Country of 1st Author: China
Year: 2017

Institute	China Agricultural University Beijing, 100193 CN
Editing Method	Embryo edit
SDN Type	SDN-3
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
AANAT aralkylamine N-acetyltransferase	The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland.	Quality	CRISPR Cas9 (spCas9)
ASMT acetylserotonin O-methyltransferase	This gene belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland.	Quality	CRISPR Cas9 (spCas9)

References, Documents, and Links

• An AANAT/ASMT transgenic animal model constructed with CRISPR/Cas9 system serving as the mammary gland bioreactor to produce melatonin-enriched milk in sheep