Species: Sterlet (Acipenser ruthenus)

Country of 1st Author: Czech Republic
Year: 2019

Institute	University of South Bohemia in Ceske Budejovice Zátiší 728/II, 389 25 Vodnany CZ
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Embryos at the 1 cell stage were injected with prepared complex of mixture all five sgRNAs and Cas9 protein (gRNAs+Cas9 ribonucleoprotein complex) in animal pole
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
dnd1 Dead end protein homolog 1	RNA-binding factor that positively regulates gene expression by prohibiting miRNA-mediated gene suppression. Relieves miRNA repression in germline cells (By similarity).	Reproduction	CRISPR Cas9 (spCas9)	Five single guide RNAs (sgRNAs) were designed to target Acipenser ruthenus dnd1 (Ardnd1) gene.

References, Documents, and Links

• Dnd1 Knockout in Sturgeons By CRISPR/Cas9 Generates Germ Cell Free Host for Surrogate Production