Species: Sheep (Ovis aries)

Country of 1st Author: New Zealand
Year: 2023

Breed Strain	Composite
Institute	AgResearch Ltd NZ
Editing Method	Embryo edit
SDN Type	SDN-2
Delivery	microinjection, plasmid, ssODN
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
NANOS2 Nanos C2HC-Type Zinc Finger 2	Germline ablated males to act as surrogate sires	Yield	CRISPR Cas9 (spCas9)	single target, HDR
DAZL Deleted In Azoospermia Like		Reproduction	CRISPR Cas9 (spCas9)

References, Documents, and Links

• Chimaeras, complementation, and controlling the male germline
• Testes of DAZL null neonatal sheep lack prospermatogonia but maintain normal somatic cell morphology and marker expression