Species: Tiger pufferfish (Takifugu rubripes)

Country of 1st Author: Japan
Year: 2019

Institute	Regional Fish Institute, Ltd. JP
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Microinjection
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
LEPR Leptin receptor	Leptin or obesity receptor (Ob-R) is a member of class I cytokine receptor family. Ob-R, expressed in six isoforms, is the product of alternative RNA splicing of db gene. According to its structural differences, the receptor's isoforms are divided into three classes: long, short, and secretory isoforms.	Yield	CRISPR Cas9 (spCas9)

References, Documents, and Links

• An effective microinjection method for genome editing of marine aquaculture fish: tiger pufferfish Takifugu rubripes and red sea bream Pagrus major