Species: Pig (Sus scrofa domesticus)

Country of 1st Author: United States
Year: 2017

Institute	University of Maryland College Park, MD 20742 US
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	The in vivo fertilized zygotes were microinjected with a mixtureof sgRNAs and a SpCas9 RNA expression construct
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
NANOS2 Nanos C2HC-Type Zinc Finger 2	Germline ablated males to act as surrogate sires	Yield	CRISPR Cas9 (spCas9)

References, Documents, and Links

• Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene