Species: Pig (Sus scrofa domesticus)

Country of 1st Author: United States
Year: 2023

Institute	Acceligen Inc. Eagan, MN US
Editing Method	Embryo edit
SDN Type	SDN-2
Delivery	single guide RNA (sgRNA) targeted the second exon, upstream of the sequence encoding the highly conserved KISS1 10-amino acid core. The designed sgRNA and Cas9 were co-injected into zygotes with two HDR templates, which separately contained a stop codon (HDRSC) and a silent blocking mutation (HDRBM). Single-stranded donor oligonucleotides (ssODNs)
Foreign DNA	Yes
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
KISS1 KiSS-1 Metastasis Suppressor	Castration free. This is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity.	Other	CRISPR Cas9 (spCas9)

References, Documents, and Links

• CRISPR/Cas9-editing of KISS1 to generate pigs with hypogonadotropic hypogonadism as a castration free trait