Species: Pig (Sus scrofa domesticus)

Country of 1st Author: Taiwan
Year: 2019

Institute	Agricultural Technology Research Institute Xiangshan Dist., Hsinchu TW
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	Two single-guide RNAs targeting the CMAH gene and Cas9 mRNA were microinjected into the cytoplasm of newly fertilized eggs
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
CMAH Cytidine monophosphate-N-acetylneuraminic acid hydroxylase	Resistance against porcine epidemic diarrhoea virus (PEDV)	Biotic Stress	CRISPR Cas9 (spCas9)	Two single-guide RNAs targeting the CMAH gene and Cas9 mRNA were microinjected into the cytoplasm of newly fertilized eggs

References, Documents, and Links

• Lessening of porcine epidemic diarrhoea virus susceptibility in piglets after editing of the CMP-N-glycolylneuraminic acid hydroxylase gene with CRISPR/Cas9 to nullify N-glycolylneuraminic acid expression