Species: Nile tilapia (Oreochromis niloticus)

Country of 1st Author: China
Year: 2017

Institute	Southwest University 400715 Chongqing CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	The gRNA and Cas9 mRNA were co-injected into one-cell stage embryos
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
eEF1A1b eukaryotic translation elongation factor 1 alpha 1b	Predicted to enable GTP binding activity; GTPase activity; and translation elongation factor activity.	Reproduction	CRISPR Cas9 (spCas9)	A guide RNA site containing BsajI adjacent to proto-spacer adjacent motif in the second exon of eEF1A1b was selected for mutation analysis

References, Documents, and Links

• Heterozygous mutation of eEF1A1b resulted in spermatogenesis arrest and infertility in male tilapia, Oreochromis niloticus