Species: Nile tilapia (Oreochromis niloticus)

Country of 1st Author: China
Year: 2019

Institute	Southwest University 400715 Chongqing CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	The guide RNA (gRNA) and Cas9 mRNA were co-injected into the one-cell stage embryos
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
esr1 Estrogen receptor 1	Estrogen receptor	Reproduction	CRISPR Cas9 (spCas9)
esr2a estrogen receptor 2a	Estrogen receptor	Reproduction	CRISPR Cas9 (spCas9)
esr2b estrogen receptor 2b	Estrogen receptor	Reproduction	CRISPR Cas9 (spCas9)

References, Documents, and Links

• Establishment of three estrogen receptors (esr1, esr2a, esr2b) knockout lines for functional study in Nile tilapia