Species: Nile tilapia (Oreochromis niloticus)

Country of 1st Author: China
Year: 2017

Institute	Guangdong Ocean University Zhanjiang 524088 CN
Editing Method	Embryo edit
SDN Type	SDN-1
Delivery	gRNA and Cas9 mRNA were co-injected into one-cell stage embryos
Foreign DNA	No
Undergone Regulatory Process	No

Target Gene(s)

Gene Name	Gene Trait	Trait Category	NGT	Methods/Remarks
wt1a	(Description of wt1a gene)	Reproduction	CRISPR Cas9 (spCas9)	The gRNA sites containing Fok I and Hp166 II adjacent to PAM, for mutation analysis, were selected in the first exon of wt1a and wt1b, respectively.
wt1b	(Description of wt1b and its function)	Reproduction	CRISPR Cas9 (spCas9)	Method specific to wt1b

References, Documents, and Links

• CRISPR/Cas9-induced disruption of wt1a and wt1b reveals their different roles in kidney and gonad development in Nile tilapia