PL1 Fusion Gene as Visual Selectable Marker in Transgenic Tomato
February 17, 2012| |
Scientists use visual selectable markers as alternative for antibiotic-resistance genes in identifying transformed cells. One example of visual selectable marker is the purple color induced by accumulation of anthocyanins. However, too much anthocyanin accumulation could impede the growth and development of transgenic plants. Thus, Feng Jin and colleagues at Nankai University in China used AtDWF4 promoter from Arabidopsis and the tomato LeANT1 gene to construct the PL1 fusion gene and analyzed if it could be used as an effective visual selectable marker gene in tomato transformation.
Results showed that all PL1 transgenic shoots showed intense purple color on shoot induction medium. Transgenic tomato plants exhibited high expression of PL1 in the true leaves and other organs of the cotyledon. They also reported that the growth and development of the transgenic plants was not affected by the expression of PL1; and conferred tolerance to multiple abiotic stresses. Using a method called "cut off green shoots", a number of transgenic tomato lines were generated with PL1 as the selectable marker gene. Therefore, PL1 could possibly be used a visual selectable marker gene for tomato transformation.
Read the research article at http://www.springerlink.com/content/72640330315j5045/.
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