Detection of Off-Target Effect in Genome Editing by Integration-Defective Lentiviral Vector
September 30, 2020| |
Guangzhou Medical University (China) and Beckman Research Institute (USA) presented an unbiased screening protocol integration-defective lentiviral vector (IDLV) and deep sequencing to map the off-target sites generated by gene editing tools. The details are published in the Methods in Molecular Biology book series.
Clustered regularly interspaced short palindromic repeat (CRISPR) and other gene editing technologies including zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) exhibit wide usage for research and therapeutics. One of the major concerns about these technologies is the off-target effects generated by nucleases at unintended genomic sequences. To address this concern, in silico methods are applied to project possible off-target sites. However, the predicted cleavage activity at these potential off-target sites does not always reflect the true cleavage in vivo with the use of available algorithms. Thus, a new screening protocol was developed by Xiaoling Wang, Youjun Wu, and Jiing-Kuan Yee.
Read more about the protocol in CRISPR Guide RNA Design.
| |
You might also like:
- Experts Review Methods Used to Minimize Off-Target Effects in Genome Editing
- Researchers Develop a Tool to Predict Off-target Editing by CRISPR
- New Software Enables Sensitive and Customized Off-target Detection for CRISPR-Cas9
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Animal Biotech Online Discourses Cover Genome Editing Regulatory Approaches
- Science and Scientists Held in High Esteem Across the World
- Review Says Farmer Knowledge Key to Finding More Resilient Crops
- Myanmar Scientists, Academics Step Up for Biotechnology Information Portal Launch
- Research Team to Genetically Improve Millet for Large-Scale Agriculture in Saudi Arabia
- Researchers Find Way to Protect Plants from Fungal Infection
-
Research Highlights
- Researchers Overexpressed Transcription Factors to Improve Cotton Lint Yield
-
Plant
- Detection of Off-Target Effect in Genome Editing by Integration-Defective Lentiviral Vector
- Gene Editing of SlLBD40 Improves Drought Tolerance in Tomato
- Researchers Engineer a Rapid TALEN Assembly Protocol for Genome Editing
-
Health
- Sewage Testing Shows Prevalence of COVID-19 Infection
- Farmers Highly Vulnerable to COVID-19, Says Study
-
Read the latest: - Biotech Updates (April 24, 2024)
- Gene Editing Supplement (April 24, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet