UConn Researcher Develops Simple, Low-Cost CRISPR-based Diagnostic Test for COVID-19April 1, 2020
Researchers at the University of Connecticut's Department of Biomedical Engineering are working to develop a new, low-cost, CRISPR-based diagnostic platform to detect infectious diseases, including the novel coronavirus (SARS-CoV-2). Associate Professor Changchun Liu developed the "All-In-One-Dual CRISPR-Cas12a" (AIOD-CRISPR) method to enable simple, rapid, ultrasensitive, visual detection of SARS-CoV-2 and HIV viruses, intended for use at home or in small clinics.
"The recent outbreak of novel coronavirus has spread rapidly all over the world," Liu says. "Rapid and early detection of the SARS-CoV-2 virus will facilitate early intervention and reduce disease transmission risk. Our method has great potential for developing next-generation point-of-care molecular diagnostics."
While the polymerase chain reaction (PCR) method is currently considered the "gold standard" for disease diagnostics, it relies on expensive equipment and well-trained personnel. Liu's method, unlike the PCR, is isothermic (~37°C), and unlike other isothermal amplification technologies, it has better sensitivity and specificity. In Liu's lab, the AIOD-CRISPR system successfully detected the DNA and RNA of SARS-CoV-2 and HIV. Additionally, the method was evaluated by detecting HIV-1 RNA extracted from human plasma samples, achieving comparable results to the PCR method.
You might also like:
- Viable Vaccine Candidate for COVID-19 Developed Using Proprietary Plant-based Technology
- Scientists Say COVID-19 Coronavirus Has Natural Origins
- Pocket K No. 54: Plant Breeding Innovation: CRISPR-Cas9
ISAAA shares, disseminates, and promotes science-based information to help in achieving global agricultural sustainability and development. During this time of COVID-19 pandemic, we monitor research on treatments, vaccines and keep track of the pandemic's effect on food security and agriculture. We help the public make informed decisions and actions to mitigate and recover from the impact of COVID-19. At this crucial time, we need your help. Please support our efforts today from as little as $10
See more articles:
- UConn Researcher Develops Simple, Low-Cost CRISPR-based Diagnostic Test for COVID-19
News from Around the World
- Study Finds Providing Facts about GMO, Climate Change to Skeptics Helps Change Their Mind
- Biotech Stacks Adoption Continues to Increase Globally
- Chile Develops Drought, Saline-Tolerant Tomato, Kiwi
- Exeter Researchers Discover Novel Chemistry Against Fungal Disease of Crops
- CBF/DREB1 Gene Family in Lettuce Confers Multiple Stress Tolerance
Plant Breeding Innovations
- CRISPR-Cas9 Reveals that OsMPT3 is a Vital Osmotic Regulatory Factor
- Knockout of the OsNAC006 TF Causes Drought and Heat Sensitivity in Rice
- Scientists Optimize Prime Editing for Rice and Wheat
Subscribe to CBU: