Experts Develop a Haploid-Inducer Mediated Genome Editing System for Faster Maize Breeding
May 2, 2019| |
Crop breeding programs aim to develop pure inbred lines with several favorable characteristics. Doubled haploid (DH) and genome editing with CRISPR-Cas9 are two powerful tools in crop breeding. However, both techniques fall short for rapid development of pure elite lines with integrated desired traits. Thus, Chinese Academy of Agricultural Sciences researchers developed a Haploid-Inducer Mediated Genome Editing (IMGE) approach, which uses a maize haploid inducer line carrying a CRISPR-Cas9 cassette targeting for a desired traits to pollinate an elite maize inbred line and to generate genome-edited haploids in the elite maize background. Through this new approach, homozygous pure DH lines with the desired trait could be generated in just two generations, avoiding the long procedure of repeated crossing and backcrossing done in conventional breeding.
Read more details from the research article in Molecular Plant.
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