Nematode Resistant Eggplant Developed Through Host-induced Silencing

RNA interference (RNAi)-based host-induced gene silencing (HIGS) is one of the new tools used in fighting phytonematode infection in crop plants. Mi-msp-1, an effector gene expressed in the subventral pharyngeal gland cells of root-knot nematode (Meloidogyne incognita) has a vital function in the parasitic process.

Scientists from Indian Agricultural Research Institute and other institutions generated six eggplant transformants with a single copy RNAi transgene of Mi-msp-1. The transgene was expressed in T1, T2 and T3 transgenic lines for which a detrimental effect on root-knot nematodes penetration, development and reproduction was documented upon challenge infection with nematode juveniles. Furthermore, the post-parasitic nematode stages derived from the transgenic plants exhibited long-term RNAi effect as manifested in the targeted downregulation of Mi-msp-1.

The results of the study imply that HIGS of Mi-msp-1 improved resistance to nematode in eggplant and protect the plant against RKN parasitism at the very early stage.

For more details, read the results published in Transgenic Research.


 

This article is part of the Crop Biotech Update, a weekly summary of world developments in agri-biotech for developing countries, produced by the Global Knowledge Center on Crop Biotechnology, International Service for the Aquisition of Agri-Biotech Applications SEAsiaCenter (ISAAA)

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