CRISPR-Cas9 Used for Targeted Disruption of FAD2–2 Microsomal Omega-6 Desaturase in SoybeanFebruary 13, 2019
RNA-guided CRISPR-Cas9 system has been regarded as a useful tool in site-directed mutagenesis because of its wide applications in various branches of biology. CRISPR-Cas9 nuclease system displaced the previously used tools and their flaws in site-specific mutagenesis. Thus, this system was used by Piwu Wang of Jilin Agricultural University and team and showed an efficient sequence specific integration/mutation of FAD2–2 gene in soybean. The results of their study was published in BMC Biotechnology.
The researchers designed a single guided RNA sequence using bioinformatics tools aimed at targeting specific portions of the FAD2-2 loci in soybean, which is vital in the biosynthesis of peak oil. Findings showed that the soybean transgenic mutants subjected to targeted mutation were detected in the target gene. Furthermore, the detection of Cas9 gene, BAR gene, and NOS terminator were carried out respectively. Further analysess confirmed the stable transformation of Cas9 gene into soybean, as well as the efficient CRISPR-Cas9/sgRNA induced mutation within the target sequence of FAD2–2 loci. FAD2–2 integration at target region in the form of substitution, deletions, and insertions were completed with notably high frequency and rare off-target mutagenesis.
The findings provides a summary of recent developments and future challenges in producing agronomically important crops using the CRISPR-Cas9 system.
Read the research article from BMC Biotechnology.
See more articles:
News from Around the World
- A Comprehensive Review of the Environmental Safety of Bt Crops Now Published
- New Chocolate has Its Own Pro-GMO Label to Promote GMO Farming
- NASA: Tree Planting and Agri in China and India Make Earth Greener
- Experts Call for Urgent Political Support for Agri-biotech in the Philippines
- Genes that Resist White Rust Pathogen Found
- Study Finds Attitudes of the Youth toward GM Food Affected by Family Members
- Rothamsted Research Applies for New Trials of GM Camelina
- COGEM: No Risk in Import and Processing of GM Maize Bt11
- Scientists Report First Genetic Transformation of Einkorn
- Haplotype Analysis of Rice Genes Helps Development of Tailor-made Rice with Improved Genetic Gains
- GMOs Not the Cause of Monarch Butterfly Decline, Study
Plant Breeding Innovations
- CRISPR-Cas9 Used for Targeted Disruption of FAD2–2 Microsomal Omega-6 Desaturase in Soybean
- Improved CRISPR System with Power on Switch for Gene Editing
Beyond Crop Biotech
- NRGene and Kayagene Introduce the First Fully-Phased Cannabis Genome
- International Conference on Industrial Crops
Subscribe to CBU: