Crop Biotech Update

CRISPR-Cas9 Used in Breeding Indica Glutinous CMS Line WX209A

December 19, 2018

Glutinous cytoplasmic male sterile (CMS) line is vital for the selection of hybrid glutinous rice combination with high yield and quality. To develop glutinous CMS with low amylose content, researcher Xin Wang and team knocked out the granule-bound starch synthase OsWaxy in 209B using CRISPR-Cas9-mediated genome editing technology and successfully obtained a glutinous maintainer line WX209B. 

When compared with maintainer line 209B, WX209B exhibited reduced amylose contents and similar agronomic characteristics. After one generation of hybridization and two generations of backcrossing with WX209B as the male parent and 209A as the female parent, the glutinous CMS line WX209A was successfully achieved. 

The results of the study provide a strategy to efficiently breed for the glutinous cytoplasmic male sterile line by combining CRISPR-Cas9-mediated gene editing technology with conventional backcross breeding method in a short period, which prepares the ground for further breeding of hybrid glutinous rice variety.

Read the research article from Czech Academy of Agricultural Sciences.