CRISPR-Edited Tomatoes with Reduced Ethylene ProductionJuly 25, 2018
Recent progress in genome editing methods has opened new avenues for reverse genetics studies in plants. One of these is the CRISPR-Cas9 system, which has been used to induce targeted mutations in a variety of organisms.
A Hankyong National University research team described a targeted mutagenesis using Agrobacterium-delivered CRISPR-Cas9 in tomato. This new system uses an Agrobacterium vector and three guide RNAs for single gene targeting. The team, which was led by Yu Jin Jung, evaluated the system for its mutagenesis frequency and heritability by testing it on the LeMADS-RIN gene of tomato.
Individual events carrying mutations in the LeMADS-RIN gene occurred in 10.6% of the transformed tomato genotypes. Compared with wildtype plants, the edited mutants exhibited more incompletely-ripening fruits and lower ethylene contents. Analysis also revealed that the desired mutant alleles are inheritable and could be retrieved in the succeeding generations.
For more information, read the article in Korean Journal of Horticultural Science & Technology.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Society Needs Radical Adaptation to Attain Nutritional Needs in 2050
- Global Biotech Seeds Market to Grow by 9.87% in 2022
- USDA APHIS Deregulates Herbicide Resistant GE Cotton
- Purdue Study Finds Key to Plant Growth Mechanism
- Iran Starts Labeling GM Food
- Filipino Farmers Losing PHP33.85 Billion Due to Delays in Commercializing Bt Eggplant
- Public Participates in Golden Rice Consultations in PH
- Scientists Restrict Bt Gene Expression in Insect Biting Sites
- Researchers Find New Candidate Genes for Disease Resistance in Tomato
Plant Breeding Innovations
- CRISPR-Edited Tomatoes with Reduced Ethylene Production
- Gene Controlling Megaspore Mitosis in Arabidopsis Found
- FZP Controls Sterile Lemma in Rice Grains
- Organizations, Scientific Leaders Send Open Letter to EC President about Plant Genome Editing
Beyond Crop Biotech
- PsFAD3 Gene Involved in ALA Accumulation in Tree Peonies
- Researchers Develop CRISPR-Cas9 Editing Techniques for Ralstonia eutropha
- Plant Genomics & Gene Editing Congress: USA
- Randy A. Hautea Memorial Fund
- Report Identifies 5 Breakthoughs to Advance Food & Agricultural Research by 2030
- Food Matters: Food Security and The Future Of Food
Subscribe to CBU: