PcWRKY33 Gene from Japanese Knotweed Reduces Salt Tolerance in Transgenic Arabidopsis
May 2, 2018| |
WRKY transcription factors play important roles in regulating biotic and abiotic stresses. University of Chinese Academy of Sciences' Wenqi Bao, along with colleagues, recently found a WRKY gene from Japanese knotweed (Polygonum cuspidatum), PcWRKY33, that encodes a nucleoprotein, which specifically binds to the promoter of target genes to regulate their expression.
Analysis shows that expression of PcWRKY33 is induced by various abiotic stresses, including salt stress and plant hormones. Overexpression of PcWRKY33 in Arabidopsis thaliana reduced its tolerance to salt stress. Specifically, a number of physiological parameters in the transgenic lines were significantly lower under salt stress.
The transgenic plants also exhibited decreased expression of stress-related genes, increased accumulation of reactive oxygen species (ROS), and decreased activities of ROS-scavenging enzymes. These suggest that PcWRKY33 negatively regulates salt tolerance by downregulating stress-related genes and increasing the level of ROS.
These results indicate that PcWRKY33 is involved in abiotic stress regulation.
For more information, read the article in Plant Cell Reports.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Borlaug Global Rust Initiative Honors Women in Wheat Research
- WEMA Maize Shows Promising Resistance to Fall Armyworm in Mozambique
- Researchers Move Toward Better Understanding of Deadly Citrus Disease
- Cotton Research Goes to Space
- Research Unlocks Rice Gene Diversity for Food Security
- Plant Scientists Boost Malaria Drug Yield in Plant
- Why Grass Cereals Are More Drought-tolerant Than Other Plants
- Europe's Current Approach to Food, Agriculture, and the Environment Not Sustainable, say European National Science Academies
-
Research Highlights
- SlAGL6 Gene is Key to Parthenocarpy in Tomato
- PcWRKY33 Gene from Japanese Knotweed Reduces Salt Tolerance in Transgenic Arabidopsis
- Gene from Wild Peanut Enhances Biotic and Abiotic Resistance in Tobacco
-
Beyond Crop Biotech
- The Effect of Single Metal Nanoparticles on Transformation Efficiency of Soybean
- FDA Approves Application for AquaBounty Salmon Facility in Indiana, USA
-
Announcements
- World Biotechnology Conference
-
Plant
- Wageningen University & Research Releases Booklet on New Plant Breeding Techniques
- CRISPR-Knockout of OsFAD2-1 Gene Improves Rice Bran Oil Health Benefits
- Japanese Researchers Establish New Cas9 Variant
- Early Leaf Senecence and Salt Stress Response Gene Found in Rice
-
Read the latest: - Biotech Updates (April 17, 2024)
- Gene Editing Supplement (April 10, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet