CRISPR Reveals the Role of SlMPK20 Protein in Tomato Pollen Development
April 25, 2018| |
Mitogen-activated protein kinases (MAPKs) regulate plant growth. However, their role in plant reproductive development remains unknown. The team from Zhejiang University in China recently discovered the role of SlMPK20, an MAPK, in pollen development in tomato (Lycopersicum esculenta).
Knock-out of SlMPK20 via CRISPR-Cas9 editing completely eliminated pollen viability in the gene-edited tomato plants with no effects on female fertility. Further analysis found that SlMPK20 plays a role during microgametogenesis. Transcriptomic and proteomic analyses also revealed that knockout of SlMPK20 significantly reduced the expression of a large number of genes controlling sugar and auxin metabolism and signaling in anthers.
These results reveal that SlMPK20 specifically regulates pollen development through modulating sugar and auxin metabolism and signaling.
For more information, read the article in The New Phytologist.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- FAO Calls for Global Coordination for a Bioeconomy that Leaves No One Behind
- CIMMYT Scientist Uses Native Maize Varieties to Find Novel Traits for Breeding
- A Scientist Works to Increase Water Saving Potential of Crops
- Earth BioGenome Project Holds Solutions for Agriculture's Future
- Agri-Biotech Project Featured in Farm Tourism Field Immersion in PH
- Fostering Innovation Key for a Healthy, Wealthy and Food-secure Commonwealth
- EuropaBio: EU Must Reinstate Science in GMO Safety Assessment; Stop Unneeded Animal Testing
- EFSA Publishes Scientific Opinion on Three‐Event Stack Cotton GHB614 × LLCotton25 × MON 15985
-
Research Highlights
- Why Rice Planthoppers Do Not Prefer Bt Rice Plants
- Transformation of Encoded GA20-oxidase Gene in Tobacco
- OsPK2 Gene Involved in Starch Synthesis and Grain Filling in Rice
-
Beyond Crop Biotech
- Research Team Creates Detailed Map of Genetic Evolution of Brewer's Yeast
- Scientists Develop Insulin-Deficient Pigs for Diabetes Research
-
Announcements
- 2018 BIO International Convention
-
Plant
- Dominant Allele Restricts Nodulation of Rhizobium Species in Soybean
- Researchers Perform Targeted Mutation and Gene Replacement in Tomato
- Application of CRISPR-Cas9 Genome Editing in Wild Strawberry
- CRISPR Reveals the Role of SlMPK20 Protein in Tomato Pollen Development
-
Read the latest: - Biotech Updates (April 24, 2024)
- Gene Editing Supplement (April 24, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet