Researchers Apply CRISPR-Cas9 on Carrot Cells
January 24, 2018| |
CRISPR-Cas9 is widely adopted in model organisms, but has not been used in carrot, an important health-promoting crop grown worldwide. Magdalena Klimek-Chodacka of the University of Agriculture in Krakow in Poland, and colleagues, reported the use of CRISPR-Cas9 for targeted mutagenesis of carrot.
Several CRISPR-Cas9 vectors that contain two single-guide RNA (gRNAs) that target the carrot flavanone-3-hydroxylase (F3H) gene were tested to block the anthocyanin synthesis in a purple-colored carrot callus. Knockout of F3H gene resulted in discoloration of calli, proving the role of F3H gene in carrot anthocyanin synthesis. This also provided a visual proof for screening successfully edited events.
This study shows the successful site-directed mutagenesis in carrot using CRISPR-Cas9 and could spark research in this important vegetable crop.
For more information, read the article in Plant Cell Reports.
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