Simultaneous Targeting of Multiple Gene Homeologs to Alter Oil Production in CamelinaMay 31, 2017
Camelina sativa can easily be genetically modified using enzymes from other plants, making it an ideal platform for the production of unusual valuable lipids. However, suppression of endogenous enzyme activity to reduce competition for substrates, such as triacylglycerol, is also required to enhance the production of target compounds. Furthermore, camelina possesses a hexaploid genome, with multiple gene homeologs coding for an enzyme.
Kansas State University researchers designed a guide RNA identical to all three CsDGAT1 or CsPDAT1 homeologs, to demonstrate the ability of the system in introducing mutations to the genes important for triacylglycerol (TAG) synthesis in developing seeds. Analysis from the edited T1 plants revealed that each CsDGAT1 or CsPDAT1 homeolog was altered by multiple mutations, resulting in a genetic mosaic in the plants.
Seeds harvested from both CsDGAT1- and CsPDAT1-targeted lines were often shrunken and wrinkled. Furthermore, lipid analysis revealed that many lines produced seed with reduced oil content and altered fatty acid composition, consistent with the role of the targeted genes.
CRISPR-Cas system therefore represents a useful method to alter endogenous biosynthetic pathways efficiently in polyploid species such as camelina.
For more on this study, read the article in Plant and Cell Physiology.
See more articles:
News from Around the World
- Global Team Led by ICRISAT Re-sequence Pigeonpea Genome
- Journalists in Uganda Urged to Support Passing of Biotech Law through Factual Reporting
- Kenya Biosafety Regulators Commend Malawi's Bt Cotton National Performance Trials
- Two New Proteins Connected to Plant Development Identified
- Draft Genome of Spinach Published by Researchers from US and China
- Research Team Discovers Gene that Impacts Soybean Nodule Development
- Engineered Rice with Adjustable Immune System Shows Resistance to Multiple Diseases at Once
- Japan, A Potential Biotech Sugar Beet Country
- Study Shows Climate Change to Cause 23% Decline in Crop Production by 2050s
- Researchers Study the Effect of Calcareous Soil on Bt Protein Expression and Efficacy in the Field
- CAAS Scientists Develop Herbicide Resistant Cotton with Low Glyphosate Residues
Plant Breeding Innovations
- CRISPR-mediated Targeted Mutagenesis in Grape
- Simultaneous Targeting of Multiple Gene Homeologs to Alter Oil Production in Camelina
Beyond Crop Biotech
- PtrMYB57 Transcription Factors Negatively Regulate Anthocyanin and Proanthocyanidin Synthesis in Poplar
- Plant-Produced Vaccine Candidate for Bluetongue Virus
Subscribe to CBU: