Pretreatment of Tea Oil Fruit Hull Using Acetic Acid Organosolv and Alkaline Hydrogen Peroxide

The tea oil fruit hull (TOFH) is mainly made up of lignocellulose and bioactive substances. Previous studies have already developed a two-stage solvent-based process, which includes an atmospheric glycerol organosolv (AGO) pretreatment, for bioprocessing of the TOFH into diverse bioproducts. However, the AGO pretreatment is not as selective as expected in removing the lignin from TOFH.

Song Tang led a team of scientists from Jiangnan University and Hunan Academy of Forestry in China, and the Queensland University of Technology in Australia to evaluate the use of acetic acid organosolv (AAO) as pretreatment for TOFH. AAO was optimized to fractionate the TOFH selectively. Alkaline hydrogen peroxide (AHP) pretreatment was then used for further delignification.

Results indicate that the AAO–AHP pretreatment had an extremely good selectivity at component fractionation, resulting in 92% delignification and 88% hemicellulose removal, with 87% cellulose retention. The pretreated substrate presented a remarkable enzymatic hydrolysis of 85%.

The AAO–AHP pretreatment can be an environmentally safe approach for pretreatment of the agroforestry biomass.


This article is part of the Crop Biotech Update, a weekly summary of world developments in agri-biotech for developing countries, produced by the Global Knowledge Center on Crop Biotechnology, International Service for the Aquisition of Agri-Biotech Applications SEAsiaCenter (ISAAA)

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