News and Trends

http://biofuels-news.com/display_news/10555/licella_and_canfor_to_form_jv_to_collaborate_on_advanced_biofuels_project/

Australia-based Licella Fibre Fuels and Canada-based Canfor Pulp Products have signed an agreement to form a joint-venture under the name Licella Pulp Joint Venture. The joint venture will study opportunities to integrate Licella's Catalytic Hydrothermal Reactor (Cat-HTR) upgrading platform into Canfor Pulp's kraft and mechanical pulp mills.

This aims to convert biomass, including wood residues from Canfor Pulp's kraft pulping processes, into biocrude oil to produce biofuels and biochemicals to further optimize their production capacity. In their trials, wood residue streams were successfully converted into a stable biocrude oil. When successful, the Licella Pulp Joint Venture would offer this solution to other Kraft and mechanical pulp mills.

Research and Development

http://www.newswise.com/articles/chance-finding-could-transform-plant-production-u-of-guelph-study

An almost entirely accidental discovery by University of Guelph researchers could transform food and biofuel production and increase carbon capture on farmland. By tweaking a plant's genetic profile, the researchers doubled the plant's growth and increased seed production.

The team studied Arabidopsis and found that inserting a particular corn enzyme caused the plant's growth rate to increase. This could boost yields of important oilseed crops such as canola and soybean, as well as crops such as camelina, which are grown for biofuels.

Their finding, however, came almost by chance. The researchers only noticed that their genetically engineered plants looked much larger than wild type plants. While genetic engineering led to more flowers and pods containing seeds, it did not alter the seed composition.


http://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0512-3

Fatty alcohols are important chemicals used in detergents, surfactants and personal care products. Biosynthesized fatty alcohol provides a promising alternative to traditional fatty alcohol industry. Harnessing microorganisms for fatty alcohol production could be a new strategy to achieve commercial production levels.

Washington State University researchers introduced a fatty acyl-CoA reductase (FAR), TaFAR1, to directly convert fatty acyl-CoA to fatty alcohol in the yeast Yarrowia lipolytica. Optimizations, including eliminating the fatty alcohol degradation pathway, enhancing TaFAR1 expression, and increasing fatty acyl-CoA supply were also conducted, resulting in 63-fold increase in the fatty alcohol-producing capability in the transgenic yeast.

This study demonstrated the development of a Y. lipolytica fatty alcohol-producing cell factor and explored fatty alcohol-producing capability through genetic modification using FAR and fatty acid metabolism.


http://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-016-0260-2

Clostridium thermocellum is an anaerobic thermophilic bacterium with the ability to produce ethanol. However, its application as biocatalyst for ethanol production is limited since pyruvate ferredoxin oxidoreductase, which directs pyruvate into the ethanol production pathway, has low affinity to the substrate. Hence, researchers from SRM University in India aimed to enhance the ethanol production of C. thermocellum.

The pyruvate carboxylase (pdc) and alcohol dehydrogenase (adh) genes from Zymomonas mobilis were cloned and transformed to Clostridium thermocellum DSM 1313 to generate recombinant CTH-pdc, CTH-adh and CTH-pdc-adh strains that carried heterologous pdc, adh, and both genes, respectively.

Although both pdc and adh were functional in C. thermocellum, the presence of adh severely limited the growth of the recombinant strains, regardless of the presence of the pdc gene. Whereas, the recombinant CTH-pdc strain showed two-fold increase in pyruvate carboxylase activity and ethanol production when compared with the wild type strain.

Pyruvate decarboxylase gene of the homoethanol pathway from Z. mobilis was functional in recombinant C. thermocellum strain and enhanced its ability to produce ethanol. Strain improvement and bioprocess optimizations may further increase the ethanol production from this recombinant strain.

Energy Crops and Feedstocks for Biofuels Production

http://link.springer.com/article/10.1007/s12155-015-9665-3

The aquatic fern Azolla is one of the fastest-growing nitrogen-fixing plants on Earth and is considered as a potential source of biomass for biofuel production. Paul Brouwer from Utrecht University led researchers in analyzing the lipid fraction from Azolla filiculoides to determine if it is fit for biodiesel production.

The harvested biomass contained 7.92 ± 0.14% dry weight (dw) crude lipids. Drying conditions did not significantly affect lipid composition or yields, indicating that it can be optimized without the risk of product loss. Extracted from the biomass were free fatty acids, fatty acid methyl esters and unique mid-chain (di)hydroxy compounds. Based on the fatty acid profile, Azolla biodiesel meets requirements set by the EN14214 standard. However, further studies are still needed to optimize biofuel production from Azolla.

Biofuels Processing

http://sitn.hms.harvard.edu/flash/2016/microorganisms-team-recycle-carbon-dioxide-biofuel/

A team from the Massachusetts Institute of Technology reports engineered microorganisms that can generate sustainable biofuel from carbon dioxide emissions. The technology being developed by Greg Stephanopoulos's group turns industrial waste gases, like carbon dioxide, into fuel.

The process starts with mixtures of carbon dioxide and either carbon monoxide or hydrogen bubbled into a two-step reactor, in which Moorella thermoacetica, bacteria found in ponds and undersea vents, consumes carbon dioxide and hydrogen for energy, forming acetic acid as a waste product. The acetic acid is then pumped into the second stage, where the modified yeast Yarrowia lipolytica, consumes the vinegar to produce lipids. These lipids can easily be converted into biodiesel.

This new process has advantages over other biofuel production methods in the availability and cost of their feedstock, or chemical ingredients. The team now plans a 20,000 L reactor, capable of producing 30-40 gallons of biodiesel per day.


http://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0533-y

Microalgae is considered a promising source for biofuel and high-value compound production. However, harvesting microalgae is a major hindrance in the algae-based processes. University of Minnesota's Aravindan Rajendran and Bo Hu tried to mimic the growth of natural lichen and develop a biofilm platform technology using filamentous fungi and microalgae to form a biofilm "mycoalgae" for easier harvest.

The possibility of co-existence of Chlorella vulgaris with various fungl cultures was first tested to identify the best strain combination for high algae harvest efficiency. Mycoalgae biofilm with algae attachment efficiency of 99.0% was achieved in a polymer-cotton composite matrix with glucose concentration of 2 g/L in the growth medium and agitation intensity of 150 rpm at 27°C. The total biomass in the co-culture with the selected strain combination (Mucor sp. and Chlorella sp.) was higher than any of the cultures tested.

Results show that algae can be grown with complete attachment to a fungal surface and can be harvested readily as a biofilm for product extraction. The mycoalgae biofilm processes and expands the scope for exploring new techniques in the bioproduction industry.


http://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0525-y

Glycoside hydrolases (GHs) and accessory proteins are key components for hydrolysis in modern biorefineries. Currently, commercialized GHs and accessory proteins are produced by ascomycetes. A team led by Fernanda Valadares from Universidade de São Paulo in Brazil now studies the proteins from wood decay fungi for their use in improving enzymatic cocktails.

GHs and accessory proteins were produced by brown-rot fungi Laetiporus sulphureus and white-rot fungi Pleurotus ostreatus. Protein extracts were then used to modify commercial cocktails for saccharification of pretreated sugarcane bagasse. The main enzymatic activities found in the wood decay fungal protein extracts were attributed to endoglucanases, xylanases and β-glucosidases.

Conversion rates were boosted when the wood decay fungal proteins were used to modify the commercial cocktails. Proteomic studies revealed 104 and 45 different proteins in the P. ostreatus and L. sulphureus extracts, respectively. The enhancement of the saccharification of pretreated substrates by the modified cocktails was attributed to GH5- and GH45-endoglucanase, GH3-β-glucosidase, and GH10-xylanase protein families.

The proteins produced by wood decay fungi provide useful tools to improve commercial enzyme cocktails currently used for the saccharification of pretreated lignocellulosic feedstock.