News and Trends

http://genome.cshlp.org/content/early/2009/10/06/gr.091777.109.full.pdf+html (Open Access Article)
http://www.thebioenergysite.com/news/4681/genome-sequence-for-biofuels-yeast

Scientists from Duke University (North Carolina, United States), together with their Brazilian research partners, reported the genome structure of a bioethanol-producing yeast that is widely used in Brazil. The organism is Saccharomyces cerevisiae PE-2 (reported to be a heterothallic diploid naturally adapted to the sugar cane fermentation process used in Brazil). Findings are reported in the journal, Genome Research (URL above). According to Dr. Lucas Argueso, lead author and researcher at Duke University's Department of Molecular Genetics and Microbiology, they took an organism (Saccharomyces cerevisiae PE-2) "that is hugely important from an industrial standpoint, but completely unknown in terms of its genetic and molecular properties". From these studies, they were able to learn more about the organization of a complex genome and how it may contribute to robust adaptation. The study suggests that industrial yeast strains may have a high rate of evolution which may enable them to adapt to stressful conditions during fermentation (for example, tolerance to increasing ethanol concentrations during fermentation). "Now we have sequenced the genome," says Dr. Argueso. "We have a road map that will allow us to build upon [the yeast's] natural abilities. This opens the door to crossing yeast strains to make even more efficient yeasts for enhanced biofuel production.".


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http://www.thebioenergysite.com/news/4632/more-corn-for-biofuels-would-hurt-water

Researchers from the Department of Agricultural and Biological Engineering, Purdue University (United States) used a modelling approach to obtain a quantitative assessment of long-term water quality impacts of land management changes associated with increased demands for corn as a transport-biofuel feedstock in the United States. Results showed that fields practicing continuous-corn rotations had lower water quality levels, compared to those which practice continuous corn-soybean rotations. The nitrogen, fungicide and phosphorus levels in water from continuous-corn rotations were higher. According to Indrajeet Chaubey, co-researcher and associate professor of agricultural and biological engineering, a shift from corn-soybean rotations to continuous corn will result in higher sediment losses. This, in turn, will "allow more fungicide and phosphorous to get into the water because they move with sediment."  The modelling results also point to "the need for additional research to fully understand the water impacts of land management decisions associated with corn grain as a feedstock for biofuel production."  More details of the study can be obtained from their paper, published in the Journal of Environmental Engineering (URL above)..


http://cleantech.com/news/5066/tuberculosis-related-bug-biofuel-po

Massachusetts Institute of Technology (MIT) scientists in the United States are trying to use the tools of synthetic biology to engineer a soil-dwelling bacterium with an ability to utilize a wide variety of feedstocks and convert them to the desired biofuel. The soil-dwelling bacterium belongs to the genus, Rhodococcus. This microorganism is known to utilize a wide variety of toxic compounds, and is also related to a type that is known to cause tuberculosis. Studies on the basic biology and chemistry related to the organism have been made, and the next step is to determine strategies to improve yields. The Cleantech website report also mentions that the scientists have created a strain of the bacteria which can utilize both glucose and xylose (the main sugars after the pretreatment and saccharification of lignocellulosic biomass) into ethanol..

Energy Crops and Feedstocks for Biofuels Production

http://www.thebioenergysite.com/news/4675/study-of-jet-fuel-made-from-saltwater-plants

The Sustainable Aviation Fuel Users Group (a consortium of stakeholder in the airline industry) has commissioned a study to assess the use of "halophytes" (plants that thrive in saline water), for large scale aviation biofuels production. The halophytes under consideration are Salicornia and saltwater mangroves. The study will be led by the Masdar Institute of Science and Technology (based in Abu Dhabi), and will be participated by the Boeing Company, Honeywell's UOP, Yale University's School of Forestry & Environmental Studies, and other academic institutions. Halophytes are seen to have advantages over other biomass-based biofuel feedstocks, because they are less resource-intensive. Halophytes have (1) the ability to thrive in arid land (will not compete with agricultural lands), and (2) can be irrigated with sea water (low water footprint). According to Billy Glover, managing director of Environmental Strategy for Boeing Commercial Airplanes, the study will determine whether "certain types of halophytes meet the carbon reduction and socioeconomic criteria that will allow them to become part of a portfolio of sustainable biofuel solutions for aviation."  It will also "evaluate aquaculture management and practices, land use and energy requirements and identify any potential adverse ecological or social impacts associated with using halophytes for energy development, specifically for aviation biofuel development.".

Biofuels Processing

http://www.biotechnologyforbiofuels.com/content/pdf/1754-6834-2-24.pdf
(Provisional PDF during time of access)

In the production of cellulose-ethanol from lignocellulosic biomass, the development of cost-effective, but highly efficient saccharifying enzymes (cellulases/hemicellulases) which act on the celluloses and hemi-celluloses of the biomass, remains an active area of research. Saccharification converts celluloses/hemicelluloses into the ethanol-fermentable sugars, glucose and xylose. A very common source of saccharifying enzymes is the fungus, Trichoderma reesei. Recently, other enzyme sources are also being explored. Scientists from the Biomass Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST, Hiroshima, Japan) compared the saccharification performance of enzymes from Trichoderma reesei and Acremonium cellulolyticus. The saccharification performances were tested on three ligncellulosic feedstocks: eucalyptus, Douglas fir and rice straw. The scientists found that culture supernatants from A. cellulolyticus had higher specific cellulose activity and higher glucose yields compared to T. reesei. However, T. reesei enzymes had a superior xylan-hydrolyzing activity (can produce more xylose) than those derived from A. cellulolyticus. Details of the research results can be accessed in the open access journal, Biotechnology for Biofuels (URL above)..

Biofuels Policy and Economics

http://www2.ademe.fr/servlet/getBin?name=72A8E00A6ABFE18953E994EFEF2CEA501255021957045 (in French) http://www.agrion.org/big_news/agrion-en-Biofuels_sugar_cane_ethanol_comes_out_top_in_French_study.htm
http://www.biofuelsdigest.com/blog2/2009/10/12/french-environmental-agency-finds-biofuels-reduce-ghg-emissions-60-80-percent/

The French Energy and Environment Management Agency (ADEME) recently released a report on the study of environmental impacts of first-generation (i.e. food-based) biofuel feedstocks that are presently used in France. Each feedstock (during its lifecycle from cultivation, transport and conversion into the biofuel) was evaluated in terms of greenhouse gas emissions, non-renewable energy consumption and impact on health. The study validates previous studies that biofuels have benefits in terms of reduction of greenhouse gas emissions. The biofuels currently used in France are reported to meet the European target for cutting carbon dioxide emissions, but performance varies, depending on the feedstock and process used to make the biofuel. Sugarcane for biofuel ethanol was ranked top on the list. The study showed that sugarcane ethanol produces 90% less greenhouse gases (relative to gasoline), and used 80% less energy during production. Director of Renewable Energy, Jean-Louis Bal, cautions however, that the study does not consider the impact of the change in function of the land used for growing crops. For example, if a forest (having trees which are capable of capturing large amounts of carbon dioxide) is cleared and converted into bioenergy crop plantations, the positive impact of the biofuel can be negated. Details of the study can be obtained from the ADEME report (URL above, in French)..