News and Trends

http://www.waste-management-world.com/articles/2015/01/shanks-to-develop-largest-organic-waste-biogas-plant-of-its-kind-in-surrey-canada.html

Iris Solutions, a consortium led by Orgaworld Canada and a part of the Organics Division of the waste and recycling firm, Shanks Group, has been selected as the proponent for the Surrey Biofuels Processing Facility project in Canada.

The facility will be designed to receive and process 115,000 tonnes of organic waste annually. When completed, it is expected to be the largest of its kind in Canada with a capacity to process 100% of the City's organic waste, along with commercial organic waste.

The facility will use an anaerobic digester to create biogas which can be used to power the city's natural gas waste collection trucks. It will also produce a compost product that will be suitable for landscaping and agricultural applications.

The facility is planned to be fully operational by late 2016.

Research and Development

http://www.biomedcentral.com/1471-2229/14/344

Lignocellulosic biomass contains high levels of pentose, making it difficult to convert into biofuels than hexose. Thus, increasing the hexose/pentose ratio in biomass is one approach for improvement. The team of Henrik V. Scheller from Joint Bioenergy Institute used genetic engineering to study if the pectic galactan levels can be increased in cell walls of Arabidopsis cells.

The team first overexpressed different plant UDP-glucose 4-epimerases (UGEs) in Arabidopsis. However, no plant UGE could increase the cell wall galactose. The team then simultaneously overexpressed AtUGE2 and the β-1,4-galactan synthase GalS1. This led to an over 80% increase in cell wall galactose in stems. Furthermore, AtUGE2 and GalS1 overexpression in combination with overexpression of the NST1 master regulator for secondary cell wall biosynthesis resulted in thicker of fiber cell walls with high galactose levels. Immunofluorescence microscopy confirmed that increased galactose was present in secondary cell walls.

Simultaneous overexpression of AtUGE2 and GalS1 increased the cell wall galactose compared to the overexpression obtained in either one, alone. The increased galactan in fiber cells also had no impact on plant development. Thus, the gene stacking approach is promising in engineering feedstocks for biofuels.


http://news.harvard.edu/gazette/story/2014/12/bacteria-churn-out-valuable-chemicals/


A team of researchers led by Harvard geneticist George Church at the Wyss Institute for Biologically Inspired Engineering and Harvard Medical School (HMS) has modified the genes of bacteria that allows them to program what chemical the cells are to produce. Their research was reported in the Proceedings of the National Academy of Sciences (PNAS).

"This advance has implications for pharmaceutical, biofuel, and renewable chemical production," said Wyss Institute Founding Director Donald Ingber.

Their technique makes the desired chemical product vital to the bacteria's survival by making it a requirement in the activation of antibiotic-resistance genes. Only the cells that generate enough of the desired chemical will be completely resistant to the antibiotic and survive to the next round of evolution.

"This is a major direction of growth in synthetic biology, where the focus has mostly been on one-off experiments until this point," said Church.


http://english.farsnews.com/newstext.aspx?nn=13931013000128

Iranian researchers from Ferdowsi University of Mashhad succeeded in producing biodiesel fuel from soya oil. They have also proposed a new method for the production of biodiesel by presenting a solid non-homogenous nanocatalyst.

In their study, which was published in Ultrasonics Sonochemistry,  a simple, cost-effective and fast method was proposed for the synthesis of biodiesel through ultrasonic waves by using potassium fluoride–gamma alumina (KF/ γ-Al2O3) nanocatalyst. The fuel was made from soya oil and has the right characteristics to be used as a fuel.

The new nanocatalyst has several advantages which include the ease of separation from the product and the possibility of its recycling and re-use.

Energy Crops and Feedstocks for Biofuels Production

http://www.biotechnologyforbiofuels.com/content/pdf/s13068-014-0183-x.pdf

Photosynthetic algae have gained attention as a feedstock for biofuel production since algae cultivation does not compete with agricultural resources. In particular, cyanobacteria are promising as a biomass feedstock due to their high photosynthetic capabilities.

A team led by Kobe University's Tomohisa Hasunuma overexpressed the flv3 gene, which encodes a flavodiiron protein involved in alternate electron flow (AEF) in photosystem I, in Synechocystis sp. PCC6803. Overexpression resulted in improved cell growth with increases in O2 evolution, intracellular ATP level, and turnover of the Calvin cycle. Further analysis confirmed that photosynthetic carbon flow was enhanced in the flv3-overexpressing strain.

Overexpression of flv3 was proven to improve photosynthesis in the Synechocystis sp. PCC6803 due to the  enhanced AEF in photosystem I.


http://link.springer.com/article/10.1007/s12155-014-9569-7/fulltext.html

Barley is a potential alternative feedstock for ethanol production since its by-product is a nutritious feed. The team of Gongshe Hu from USDA Agricultural Research Service tested a barley mutant, m351, possessing a lower beta-glucan content, for its ethanol production efficiency and feed fraction quality.

Grain samples from m351 were used for ethanol production and produced the same amount of ethanol as the wild types. However, the feed fraction derived from the fermentation process of the m351 mutant showed 5% more protein and 7% less beta-glucan than that from the wild type, indicating more valuable feed fraction from m351.

The experiment provided evidence that m351 is a useful genetic resource for developing barley cultivars for use in bioethanol and feed production.

Biofuels Processing

http://www.waste-management-world.com/articles/2015/01/biofuel-from-pressure-cooked-wet-farm-waste-at-canadian-university.html

Researchers at the University of Guelph in Ontario, Canada have developed a procedure to produce biofuel from farm waste that is typically difficult to use. Led by engineering professor Animesh Dutta from the University of Guelph, the researchers have found a solution to this: pressure cooking.

Cooking farm waste yields compact, easily transportable material that will not degrade and can be used in energy recovery plants. The new biofuel product  made by the researchers is claimed to contain less alkali and alkaline earth metals, allowing them to be used at power plants.

"What this means is that we have a resource in farm waste that is readily available, can produce energy at a similar level to burning coal, and does not require any significant start-up costs," Dutta said.

"We are taking what is now a net-negative resource in farm waste, which farmers have to pay to remove, and providing an opportunity for them to make money and help the environment. It's a closed-loop cycle, meaning we don't have to worry about external costs," continued the professor.


http://www.waste-management-world.com/articles/2015/01/algae-to-recycle-metals-and-biofuel-from-contaminated-mine-water-in-cornwall.html

University of Bath researchers are developing a process in algae that will be used to clean up contaminated water at a former tin mine, while also recycling heavy metals as well as produce biofuels.

Researchers are to take untreated mine water samples from Wheal Jane tin mine in Cornwall into the laboratory and grow algae in them. The research will explore if algae is effective in removing toxic materials from the mine water. The project will also study the conversion of the algae into a solid from which precious heavy metals could possibly be extracted and recycled. The remaining solid waste will then be used to make biofuels.

"It's a win-win solution to a significant environmental problem. We're putting contaminated water in and taking out valuable metals, clean water and producing fuel," commented Dr. Chris Chuck, a research fellow from the University of Bath's Centre for Sustainable Chemical Technologies. "This technology could be applied to any type of mine or could even be used to clean up industrial effluent in the future," continued Chuck.