CROP BIOTECH UPDATE
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A weekly summary of world developments in agri-biotech for developing countries, produced by the Global Knowledge Center on Crop Biotechnology, International Service for the Acquisition of Agri-biotech Applications SEAsiaCenter (ISAAA)
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July 1, 2026

In This Week’s Issue:

News

New Breeding Technologies
• CRISPR Reveals Genetic Pathway for Waterlogging Tolerance in Cucumber



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NEWS
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New Breeding Technologies
CRISPR REVEALS GENETIC PATHWAY FOR WATERLOGGING TOLERANCE IN CUCUMBER

Researchers have identified a gene regulatory network that enables cucumber plants to better tolerate waterlogging by promoting the growth of adventitious roots, which help plants absorb oxygen under flooded conditions. The study found that the transcription factor CsWRKY41 plays a central role in this process by linking ethylene and abscisic acid (ABA) signaling.

The researchers developed CRISPR-Cas9-edited cucumber plants lacking the CsWRKY41 gene, as well as plants that overexpressed it. The results showed that disabling CsWRKY41 significantly reduced adventitious root formation, while increasing its expression enhanced root development. Further analyses revealed that CsWRKY41 regulates the ABA-related genes CsPYL4 and CsPP2C24, which work together to control stress responses.

The findings showed that CsPYL4 also increased the activity of the CsPrx2 gene and promoted hydrogen peroxide accumulation, both of which supported adventitious root growth. The researchers said the newly identified CsWRKY41-CsPYL4-CsPP2C24 pathway could serve as a promising target for breeding cucumber varieties with improved tolerance to waterlogging stress.

For more information, read the abstract from Plant Physiology.