News and Trends

http://biofuels-news.com/display_news/10028/shell_india_to_build_new_demo_biofuel_plant_in_bangalore/

Shell India Markets, the Indian arm of Royal Dutch Shell, has plans to build a biofuel demonstration plant using IH2 technology in Bangalore, India.

IH2 technology is a continuous catalytic thermo-chemical process which converts a broad range of forestry or agricultural residues and municipal wastes directly into renewable transportation fuels and blend stocks. As the IH2 technology converts a wide range of residual biomass, the facility in Bangalore will be designed to allow variation in feedstock.

According to Shell India, highly relevant commercial feedstock, such as residual woody biomass and agricultural and municipal residues, will be within the intended feed scope.


http://biofuels-news.com/display_news/10010/new_zealands_first_commercial_biodiesel_plant_set_for_testing/

Z Energy, a New Zealand energy producer, has moved towards completing New Zealand's first commercial scale biodiesel plant in Wiri, Auckland, with the development of a biodiesel distillation column.

The plant will turn inedible tallow, a by-product of the New Zealand meat industry, into biodiesel with the potential to scale production up. Z Energy says the biodiesel distillation column is a sophisticated piece of kit which will ensure a highly refined, pure finished biodiesel.

Fuel burned for transportation makes up around 17% of New Zealand's greenhouse gas emissions. According to David Binnie, Z's general manager of supply and distribution, it will take time, but the investment is a step towards reducing New Zealand's reliance on fossil fuels.

The biodiesel will be available to customers towards the middle of 2016.

Research and Development

http://www.upi.com/Science_News/2016/01/05/Novel-nano-reactor-pumps-out-hydrogen-biofuel/8371452003034/

By inserting a modified enzyme inside a virus, researchers, led by Trevor Douglas from Indiana University, have created a new biomaterial capable of catalyzing the production of hydrogen. The bacteriophage P22, a bacterial virus, could lead to more efficient and profitable biofuel production processes.

Researchers created the modified enzyme by extracting two genes, hyaA and hyaB, from Escherichia coli which encode subunits of the hydrogenase enzyme. Once inside the capsid, the protective protein shell of a virus, the bacteriophage P22 is born. Inside the capsid, the hydrogen-producing enzyme is 150 times more efficient than an unaltered enzyme.

Researchers have previously tried to use the enzyme to produce biofuels, but in its unaltered form it is overly sensitive to heat and outside chemicals. The enzyme becomes resilient once inside the virus shell.


http://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-015-0408-7

Clostridium pasteurianum can produce butanol from glycerol, a waste product of biodiesel production. Nicholas R. Sandoval and his colleagues from the University of Delaware in the United States aim to couple butanol fermentation with biodiesel production to improve the economic viability of biofuels. However, crude glycerol contains growth-inhibiting by-products which inhibit production.

A culture of wild-type C. pasteurianum (ATCC 6013) was chemically-mutated, and the resulting population underwent selection in increasing concentrations of crude glycerol. The best-performing mutant, M150B, showed a 91% increase in butanol production compared to wild-types.

Mutations were identified in the M150B genome including a deletion mutation in the gene of the master transcriptional regulator of sporulation, Spo0A. A spo0A-single gene knockout strain was then developed and showed similar tolerance to crude glycerol as the evolved mutant strain M150B.

Growth-associated butanol production shows C. pasteurianum to be an attractive option for further engineering as it could be a good candidate for butanol production.

Energy Crops and Feedstocks for Biofuels Production

http://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-015-0414-9

Low cost of raw materials and good process yields are necessary for future lignocellulosic biomass biorefineries. With the seasonal availability of wheat straw and the year round availability of hybrid poplar in the Pacific Northwest, researchers from University of Washington, led by Rodrigo Morales Vera, aims to determine the effect of mixing wheat straw and hybrid poplar as feedstock on the overall sugar production.

After steam pretreatment and saccharification, the mixture showed higher sugar yields than that produced from hybrid poplar or wheat straw alone. Analysis revealed that blending hybrid poplar and wheat straw resulted in more monomeric sugar recovery and less sugar degradation.

This synergistic effect was due to the interaction of hybrid poplar's high acetic acid content and the presence of ash supplied by wheat straw. Combining the two enables sourcing of cheap biomass, reduces seasonal dependency, and results in increasing biofuels and chemicals productivity.


http://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-015-0404-y

Jatropha (Jatropha curcas L.) is a biodiesel crop that can be cultivated on marginal land. However, its seed yield remains low prompting improvements on its seed productivity. Researchers from the National University of Singapore, led by Munusamy Madhaiyan, investigated nitrogen-fixing bacteria in Jatropha to exploit plant–microbe interactions and improve productivity.

Bacterial isolates from Jatropha were evaluated and Methylobacterium species accounted for 69.1% of bacteria in leaves, majority of which were nitrogen-fixing. A Methylobacterium isolate, strain L2-4, was then selected and studied.

Foliar spray of L2-4 led to successful colonization of the surface and internal tissues of leaves. It significantly improved plant height, leaf number, chlorophyll content and stem volume of plants. It also increased female–male flower ratio in plants, resulting in a seed yield increase.

This bacteria–plant interaction may significantly contribute to Jatropha's tolerance to low soil nutrient content. Strain L2-4 opens a new possibility to improve plant's nitrogen supply from the leaves and may be exploited to significantly improve Jatropha's productivity.

Biofuels Processing

http://pubs.acs.org/doi/abs/10.1021/acs.energyfuels.5b01671?source=cen&

Hydrothermal carbonization (HTC) is a technique that converts wet biomass into a coal-like material and has recently been used to treat complex waste streams, a mixture of lignocellulosic and non-lignocellulosic biomass, such as sewage. However, there is limited knowledge on the effectiveness of HTC on purely non-lignocellulosic waste such as seafood waste.

Scientists led by Shrikalaa Kannan of the McGill University in Canada developed a strategy to use pretreated seafood waste for HTC to produce hydrochar and biocrude liquor. Hydrochar and biocrude liquor were then generated from hydrolyzed fish and shrimp waste by microwave hydrothermal carbonization (MHTC).

The study marks the first time that MHTC was successfully done to produce valuable products from pure non-lignocellulosic waste like seafood waste. This would pave the way for effective use of other moisture-rich non-lignocellulosic industrial wastes for biofuel production.


http://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-015-0419-4

Efficient and cost-effective production of bioethanol from lignocellulosic biomass depends on the development of a suitable pretreatment system. Chonnam National University's Seung Gon Wi and Eun Jin Cho led a team of researchers in investigating a new pretreatment method for downstream biocatalytic hydrolysis of lignocellulosic biomass, which can accelerate bioethanol commercialization.

The optimal conditions for the hydrogen peroxide–acetic acid (HPAC) pretreatment were two hours at a temperature of 80°C with the biomass mixed with equal volumes of hydrogen peroxide and acetic acid. Compared to other pretreatments at the same conditions, HPAC was more effective at increasing enzymatic digestibility of the biomass. The pretreatment removed 97.2% of the lignin, resulting in a more efficient conversion of hydrolyzates into ethanol.

The newly developed HPAC pretreatment was highly effective for removing lignin from cell walls, resulting in enhanced enzymatic accessibility of the substrate and more efficient cellulose hydrolysis.