Glyphosate Tolerance in GM Canola by a Modified gox Gene
August 3, 2012| |
Glyphosate is the most widely used herbicide globally due to its minimal human and environmental toxicity. Application of glyphosate degrading enzyme from bacterium such as glyphosate oxidoreductase (GOX) together with glyphosate tolerant epsps is an effective technique to provide maximum glyphosate tolerance in important crops. Thus, a team of scientists led by Faranak Hadi from the National Institute of Genetic Engineering and Biotechnology (NIGEB) in Iran used a synthetic gene encoding GOX enzyme with plant codon preferences.
The team used bioinformatics tools to analyze the structure of the synthetic construct and its mRNA. They subcloned the synthetic gene and transformed it into canola through Agrobacterium mediated transformation to study further the possible functions of the gene in boosting glyphosate tolerance. Analyses confirmed the presence and the expression of the gene in the plant. When exposed to different concentrations of glyphosate, it was shown that the transgenic canola tolerated the herbicide at 1.5 mM concentration while the non-GM plant was unable to survive even at 0.5mM concentration.
Download a copy of the research paper at http://journals.ut.ac.ir/page/download-azLuMfJ8t0M.artdl.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- FAO Director-General Calls on Academia in Fight Against Hunger
- IFPRI Modeling Study Shows Which Technologies Provide Significant Yield Improvement
- IITA releases Vitamin A-enriched Maize in Nigeria
- World Bank Report Urges African Countries to Harmonize Biosafety Regulation
- South Africa GM Maize Gets Commodity Clearance
- Kenyan Farmers Benefit from Customized Maize Seeds for Dryland Areas
- Drought Tolerant Corn Shows Promising Results
- Giving Winter Wheat Research a Boost with a $475,000 investment in Canada
- Conservation Tillage Saves Oil, Soil and Toil in Cotton
- US Gov't Funds Research on Shrub Willow as Biofuel Potential
- IRM's Contribution to Stewardship of Bt Crops
- Australian Research Partnership for Increasing Wheat Yield
- OGTR Issues License for Environmental Release of GM Cotton
- GRDC Releases Five Year Strategic Plan 2012-2017
- Tel Aviv Researcher Says Plants can See, Smell, Feel, and Taste
- National Gene Bank at NIB Bangladesh
- TEAGASC to Investigate Environmental Impact of GM Blight Resistant Potatoes
- ISGA Farmers Share Biotechnology Experience with French Stakeholders
- Glover: GE Food Poses No Risk
- GM Feeding Studies Show Bt Maize OK for Pigs
- Gene Technology Helps Deceive Greedy Pest Insects
-
Research Highlights
- Glyphosate Tolerance in GM Canola by a Modified gox Gene
- Suppression of Pink Bollworm on Non-Bt Cotton by Bt Cotton in China
- Effect of Cleistogamy in GM Rice Agronomic Traits and Gene Containment
-
Beyond Crop Biotech
- Unearthing the Plant-Microbe Interaction Mystery
- First Complete Computer Model of an Organism Released by Stanford
-
Announcements
- 2nd Global Conference on Agriculture, Food Security and Climate Change
-
Resources
- How Do "Imagined Farmers" Negotiate Actual Risks?
- CCAFS Annual Report Now Available
- Global Wheat Rust Monitoring Website
- Agricultural Innovation Systems and Family Farming Summary Report
- Online Knowledge Bank to Help Farmers Fight Crop Pests and Diseases
-
Read the latest: - Biotech Updates (April 24, 2024)
- Gene Editing Supplement (April 24, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet